The critical storage protocols, specifically refrigeration and the 28-day rule, only begin *after* you have opened the vial for the first time
Patients with these risk factors should discuss screening and supplementation strategies with their GP, particularly if experiencing symptoms such as fatigue, paraesthesia, glossitis, or cognitive changes suggestive of deficiency
It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile
Nonetheless, some peptides/proteins have advanced through drug development, as shown in Table 4
